N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis

N-Acetylcysteine Suppresses LPS-Induced Pathological Angiogenesis

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Background/Aims: Angiogenesis is a key feature during embryo development but is also part of the pathogenesis of cancer in adult life.Angiogenesis might be modulated by inflammation.Methods: We established an angiogenesis model in chick chorioallantoic membrane (CAM) induced by the exposure of lipopolysaccharide (LPS), and analyzed the effects of the antioxidant N-acetylcysteine (NAC) on angiogenesis in this model as well as on the expression of key genes known to involved in the regulation of angiogenesis.Results: Treatment with NAC was able to normalize iphone 14 price chicago LPS iphone 13 price ohio induced angiogenesis and restore the LPS-induced damage of vascular epithelium in chick CAM.Using quantitative PCR, we showed that NAC administration normalized the LPS induced expression of Keap1-Nrf2 signaling and oxidative stress key enzyme gene expressions (SOD, GPx and YAP1).

Conclusion: We established a LPS-induced angiogenesis model in chick CAM.NAC administration could effectively inhibit LPS-induced angiogenesis and restore the integrity of endothelium on chick CAM.LPS exposure caused an increased expression of genes involved in oxidative stress in chick CAM.NAC administration could abolish this effect.